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Control of seed-to-seedling transition by an upstream open reading frame in ABSCISIC ACID DEFICIENT2

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA. 2025-06; 
Zhen Wang , Xiaofan Zhang , Can Zhou , Xiaofeng Cao
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Gene Synthesis The corresponding 5′ UTR sequences from ABA2 of Arabidopsis or rice were synthesized by GenScript company (at Nanjing, China) and then cloned into a dual-luciferase assay reporter construct upstream of the FLUC reporter and downstream of the UBIQUITIN promoter. Get A Quote

摘要

The start of seed germination is a major decision point in plant life cycle, which relies on seed stored mRNA. However, the underlying translational mechanism remains less illustrated. Here, we demonstrate that inhibiting translation using translation inhibitors and ribosome-defective mutants delays germination in Arabidopsis. Through comprehensive transcriptome deep sequencing (RNA-seq) and polysome profiling analyses, we elucidated the dynamic interplay of regulation at the transcriptional and translational levels during germination. We show that delayed germination in some ribosome-defective mutants is partially regulated by the gene ABSCISIC ACID DEFICIENT2 (ABA2), with an upstream open reading frame (uORF)... More

關鍵詞

ABA; preharvest sprouting; seed dormancy and germination; translation; uORF.
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