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Crystal structure of the SH3 domain of growth factor receptor-bound protein 2

Acta Crystallogr F Struct Biol Commun. 2020;

Alexandr Bolgov, Svetlana Korban, Dmitrii Luzik, Vladimir Zhemkov, Meewhi Kim, Olga Rogacheva, Ilya Bezprozvanny

Products/Services Used	Details	Operation
Proteins, Expression, Isolation and Analysis	… 2. Materials and methods 2.1. Protein expression and purification A pET-28a(+) vector for the SH3N-Grb2 C32S construct encoding residues 1–59 of human Grb2 and an N-terminal thrombin-cleavable 6?His tag were purchased from GenScript …	Get A Quote

摘要

This study presents the crystal structure of the N-terminal SH3 (SH3N) domain of growth factor receptor-bound protein 2 (Grb2) at 2.5?? resolution. Grb2 is a small (215-amino-acid) adaptor protein that is widely expressed and involved in signal transduction/cell communication. The crystal structure of full-length Grb2 has previously been reported (PDB entry 1gri). The structure of the isolated SH3N domain is consistent with the full-length structure. The structure of the isolated SH3N domain was solved at a higher resolution (2.5?? compared with 3.1?? for the previously deposited structure) and made it possible to resolve some of the loops that were missing in the full-length structure. In addition, in... More