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Deployable CRISPR-Cas13a diagnostic tools to detect and report Ebola and Lassa virus cases in real-time

Nat Commun. 2020;

Kayla G Barnes, Anna E Lachenauer, Adam Nitido, Sameed Siddiqui, Robin Gross, Brett Beitzel, Katherine J Siddle, Catherine A Freije, Bonnie Dighero-Kemp, Samar B Mehta, Amber Carter, Jessica Uwanibe, Fehintola Ajogbasile, Testimony Olumade, Ikponmwosa Odia, John Demby Sandi, Mambu Momoh, Hayden C Metsky, Chloe K Boehm, Aaron E Lin, Molly Kemball, Daniel J Park, Luis Branco, Matt Boisen, Brian Sullivan, Mihret F Amare, Abdulwasiu B Tiamiyu, Zahra F Parker, Michael Iroezindu, Donald S Grant, Kayvon Modjarrad, Cameron Myhrvold, Robert F Garry, Gustavo Palacios, Lisa E Hensley, Stephen F Schaffner, Christian T Happi, Andres Colubri, Pardis C Sabeti

Products/Services Used	Details	Operation
Proteins, Expression, Isolation and Analysis	… 11 Center for Genome Sciences, The United States Army Medical Research Institute for Infectious Disease, 1425 Porter St., Ft. Detrick, MD 21702, United States. 12 Center for Systems Biology, Department?… LwaCas13a was purified by Genscript. For the EBOV?…	Get A Quote

摘要

Recent outbreaks of viral hemorrhagic fevers (VHFs), including Ebola virus disease (EVD) and Lassa fever (LF), highlight the urgent need for sensitive, deployable tests to diagnose these devastating human diseases. Here we develop CRISPR-Cas13a-based (SHERLOCK) diagnostics targeting Ebola virus (EBOV) and Lassa virus (LASV), with both fluorescent and lateral flow readouts. We demonstrate on laboratory and clinical samples the sensitivity of these assays and the capacity of the SHERLOCK platform to handle virus-specific diagnostic challenges. We perform safety testing to demonstrate the efficacy of our HUDSON protocol in heat-inactivating VHF viruses before SHERLOCK testing, eliminating the need for an extractio... More