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| Recombinant Proteins | Undifferentiated mESCs were cultured based on previously reported methods by Shen and Qu (23). Briefly, PBS with 1% gelatin was poured into 96- well culture plates and incubated for 30 minutes at room temperature. Excess gelatin was removed by aspiration and the cells were rinsed with PBS. mESCs were suspended at a density of 1-3×105?in knockout Dulbecco’s modified Eagle medium (KO-DMEM, Gibco, USA) with 20% (v/v) heatinactivated FBS, 100 U/ml penicillin (Gibco, USA), 100 mg/ml streptomycin (Gibco, USA), 2 mM 2-mercaptoethanol (Sigma, USA), 0.1 mM nonessential amino acids (Sigma, USA), 2 mM L-glutamine (Sigma, USA) and 10 ng/ml murine recombinant leukemia inhibitory factor (GenScript USA, Inc., USA, mESC cell medium). Cells were incubated under conditions described above. The third passage of cells was used for all experiments. | Get A Quote |
Objective Superparamagnetic iron oxide nanoparticles (SPIONs) have been used to label mammalian cells and to monitor their fate in vivo using magnetic resonance imaging (MRI). However, the effectiveness of phenotype of labeled cells by SPIONs is still a matter of question. The aim of this study was to investigate the efficiency and biological effects of labeled mouse embryonic stem cells (mESCs) using ferumoxide- protamine sulfate complex. Materials and Methods In an experimental study, undifferentiated mESCs, C571 line, a generous gift of Stem Cell Technology Company, were cultured on gelatin-coated flasks. The proliferation and viability of SPION-labeled cells were compared with control. ESCs and embryoid ... More
