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Molecular cloning, characterization and expression analysis of BcHHP3 under abiotic stress in Pak-choi (Brassica rapa ssp Chinensis)

Journal of Plant Interactions. 2018;

Jin Wang, Feiyi Huang, Xiong You?&Xilin HouState Key Labo

Products/Services Used	Details	Operation
Catalog Antibody	To measure the changes of BcHHP3 expression under diverse stress treatments, we performed real-time quantitative PCR (qRT-PCR). The qRT-PCR primers were designed by the online primer design tool from the Genscript Biotechnology Company (https://www.genscript.com/ssl-bin/app/primer) and are listed in?Table 1. From the five-leaf stage seedlings of Pak-choi (B.?rapa?ssp.?chinensis?cv.?	Get A Quote

摘要

In this study,?BcHHP3?was isolated from Pak-choi; it has an open-reading frame (ORF) of 1044 base pairs, encoding 347 amino acids, a molecular weight of 39.35?kDa, isoelectric point (pI) of 9.08, an instability index of 48.35, and grand average of hydropathicity of 0.382. Multi-alignment and phylogenetic analysis showed that?BcHHP3?bears a high similarity to?AtHHP2. As predicted by SOMPA and SWISS-MODEL databases, the structure of the BcHHP3 protein is relatively stable and highly conservative. Real-time quantitative polymerase chain reaction (qRT-PCR) analysis showed that BcHHP3 was induced to co-express under cold and abscisic acid (ABA) stresses. The BcHHP3-GFP fusion protein was localized on the cell... More