| Products/Services Used | Details | Operation |
|---|---|---|
| Catalog Antibody | MTT [3-(4, 5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide and?dimethyl sulfoxide?(DMSO) were purchased from Sigma Chemical Co. (USA). Dulbecco's modified Eagle’s medium (DMEM) was obtained from Invitrogen (USA). The primers of?caspase-3?for?real-time PCR?were purchased from Genscript (China). Antibodies against Caspase-3 were obtained from Cell Signaling Technology (USA). | Get A Quote |
Araucaria?is a small genus of evergreen coniferous trees, widely used for ornamental and timber purposes.?Araucaria?species are rich in essential oils. In this study, the essential oils obtained from the foliage of?Araucaria heterophylla?(Salisb.) and?Araucaria bidwillii?Hook were analyzed using GC and GC/MS. Cytotoxicity of both essential oils was assessed on three human cancer cell lines using MTT assay. Twenty nine compounds were annotated in?A. heterophylla?essential oil, whereas forty three were identified in?A. bidwillii?accounting for 95.16% and 81.06% of the total volatile oil composition, respectively. α-Pinene (70.85%),?d-limonene (4.26%) and germacrene D (2.99%) represented the major com... More
