| Products/Services Used | Details | Operation |
|---|---|---|
| Proteins, Expression, Isolation and Analysis | Images culture medium supplemented with M-CSF (25 ng/mL, Genscript), 10% human AB Monocyte-derived macrophages (MDMs) were also purified from human blood samples serum (Valley Biomedical) and 1% penicillin-streptomycin solution (GIBCO Life fully automated inverted Leica DMI6000 B microscope (Leica Microsystems) or a Fluorescence microscopy. | Get A Quote |
Extracellular traps ejected by various immune cells (neutrophils, macrophages, eosinophils and mast cells) have several immune functions, either protective against pathogens or deleterious in some autoimmune or inflammatory disorders. Since their first description in 2004, the mechanisms of extracellular traps formation have been extensively investigated though still not fully understood. We describe here a new tool for the detection of extracellular traps by fluorescence microscopy in a single-step staining protocol, which does not require any wash. The approach uses the GreenGlo? DNA dye, which can differentiate between nuclear DNA and extracellular DNA (extracellular traps) released from cells using differ... More
