Malate?dehydrogenases?(Mdhs)?reversibly?convert?malate?to?oxaloacetate?and serve?as?important?enzymes?in?several?metabolic?pathways.?In?the?yeast? Saccharomyces cerevisiae?there?are?three?Mdh?isozymes,?localized?to?different compartments?in?the?cell.?In?order?to?identify?specifically?the?Mdh2?isozyme, GenScript?USA?produced?three?different?antibodies?that?we?further?tested?by western?blot.?All?three?antibodies?recognized?the?S. cerevisiae?Mdh2?with different?background?and?specificity?properties.?One?of?the?antibodies?had?a relatively?low?background?and?high?specificity?and?thus?can?be?used?for?specific identification?of?Mdh2?in?... More