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Expression, purification and crystallization of an SLC16 monocarboxylate transporter family homologue specific for l-lactate.

Protein Expr Purif. 2020;

Bonetti S1, Hirschi S1, Bosshart PD2.

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Codon Optimization	… From the obtained genes, the codon-optimized version of a major facilitator superfamily-type<br> transporter from Syntrophobacter fumaroxidans (SfMCT, UniProt ID code A0LNN5) was<br> synthesized for expression in Escherichia coli (<b>GenScript</b>) containing 5′-HindIII and 3′-XhoI …	Get A Quote

摘要

l-lactate plays an important role as metabolite and signaling molecule in eukaryotes and bacteria. Monocarboxylate transporters (MCTs) of the SLC16 solute carrier family are responsible for the transport of l-lactate across eukaryotic and bacterial cell membranes. Here we report an efficient protocol for the expression and purification of an SLC16 family homologue in milligram amounts. The purified protein is stable and can thus be used for biochemical and structural studies as shown by successful crystallization.,Copyright ? 2019 Elsevier Inc. All rights reserved.

關(guān)鍵詞

Expression of membrane proteins; Membrane protein; Monocarboxylate transporter; Purification of membrane proteins; SLC16 family; Structure; l-lactate transporter

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