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Basal Spontaneous Firing of Rabbit Sinoatrial Node Cells Is Regulated by Dual Activation of PDEs (Phosphodiesterases) 3 and 4.

Circ Arrhythm Electrophysiol. 2018;

VinogradovaTatiana M，SirenkoSyevda，LukyanenkoYevgeniya O，YangDongmei，TarasovKirill V，LyashkovAlexey E，VargheseNevin J，LiYue，ChakirKhalid，ZimanBruce，LakattaEdwa

Products/Services Used	Details	Operation
Catalog Antibody	PDE3A, PDE4A, PDE4B, and PDE4D in the sinoatrial node and LV were detected using PDE3A, PDE4A, and PDE4D antibody (Abcam, Cambridge, MA) and custom-made PDE4B antibody (GenScript, Piscataway, NJ). PDE3A was detected with PDE3A antibodies (Abcam, ab99236) followed by secondary goat-anti-rabbit antibody (Jackson ImmunoResearch Laboratories, 111- 035-047); PDE4A was detected using PDE4A antibody (Abcam, ab14607) followed by secondary mouse-anti-rabbit antibody (Jackson ImmunoResearch Laboratories, 211-032-171); for PDE4B we used custom made antibody (GenScript) with secondary goat anti-mouse antibody (Jackson ImmunoResearch Laboratories, 115-035-072); PDE4D was detected using PDE4D antibody (Abcam, ab77080) with secondary donkey anti-goat antibody (SantaCruz, sc-2020 or Abcam, ab97110). Cells were 5 incubated with primary antibody anti-PDE3A (Abcam, ab99236, 1:100) together with either anti- ?-actinin (Sigma-Aldrich A7811, 1:500) or anti-PDE4B (GenScript custom 9G9E2, 1:100,) or anti-SERCA2 (ThermoFisher MA3-910, 1:100) or anti-PLB antibodies (Badrilla A010-14, 1:200).	Get A Quote

摘要

Spontaneous firing of sinoatrial node cells (SANCs) is regulated by cAMP-mediated， PKA (protein kinase A)-dependent (cAMP/PKA) local subsarcolemmal Ca releases (LCRs) from RyRs (ryanodine receptors). LCRs occur during diastolic depolarization and activate an inward Na/Ca exchange current that accelerates diastolic depolarization rate prompting the next action potential. PDEs (phosphodiesterases) regulate cAMP-mediated signaling; PDE3/PDE4 represent major PDE activities in SANC， but how they modulate LCRs and basal spontaneous SANC firing remains unknown.