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Soluble expression of recomb inant cMyc， Klf4， Oct4， and Sox2 proteins in bacteria and transduction into living cells.

Int J Ophthalmol. 2017-01;

LiuGuo-Dan, ZhouShi-Feng, DingXu-Chen, FangChun-Lai, MiShu-Yong, GaoXiang-Chun, Han

Products/Services Used	Details	Operation
Catalog Antibody	… Fetal Bovine Serum (FBS), Dulbecco's Modified Eagle Medium (DMEM), ampicillin, chloramphenicol, and Alexa488-conjugated secondary antibody were purchased from Invitrogen (Carlsbad, CA, USA). TAT and NLS sequences were synthesized at GenScript (Nanjing, China) …	Get A Quote

摘要

To develop a new method to produce recombinant reprogramming proteins， cMyc， Klf4， Oct4， and Sox2， in soluble format with low cost for the generation of induced pluripotent stem cells (iPSCs).