| Products/Services Used | Details | Operation |
|---|---|---|
| Recombinant Proteins | … HGF) (ProSpec, USA), and 10 ng/ml fibroblast growth factor-4 (FGF-4) (Millipore, USA) for 2 weeks followed by treatment with step-two maturation medium, consisting of DMEM supplemented with 5% FBS, 20 ng/ml HGF, 20 ng/ml oncostatin-M (OSM) (Genscript, USA), 10 ?6 M … | Get A Quote |
Resistance to oxidative damages in undifferentiated mesenchymal stem cells (MSCs) in comparison with the undifferentiated progenitor cells may differ depending on several factors. This study was carried out to examine the impact of hepatogenic differentiation process of MSCs on oxidative DNA damage markers. Hepatic differentiation of MSCs was carried out using a two-step conventional protocol and the cells were processed for characterization using morphological and biochemical markers. During the course of differentiation cellular levels of reactive oxygen species (ROS)， 8-hydroxy-2'-deoxyguanosine (8-OH-dG) and expression of ataxia-telangiectasia mutated (ATM) protein were estimated at time intervals (10， ... More
