| Products/Services Used | Details | Operation |
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| Catalog Peptides | After centrifugation, the supernatant was incubated with anti-DYKDDDDK G1 Affinity Resin (GenScript) for 3 h at 4 °C. The complex sample was eluted in a buffer containing 20 mM HEPES (pH 7.4), 150 mM NaCl, 0.01% (w/v) LMNG, 0.002% CHS, 10 μM ligand, and 0.2 mg/mL Flag peptide (GenScript). | Get A Quote |
Gout, a common and painful disease, stems from hyperuricemia, where elevated blood urate levels lead to urate crystal formation in joints and kidneys. The human urate transporter 1 (hURAT1) plays a critical role in urate homeostasis by facilitating urate reabsorption in the renal proximal tubule, making it a key target for gout therapy. Pharmacological inhibition of hURAT1 with drugs such as dotinurad, benzbromarone, lesinurad, and verinurad promotes urate excretion and alleviates gout symptoms. Here, we present cryo-electron microscopy structures of native hURAT1 bound with these anti-gout drugs in the inward-open state, and with urate in inward-open, outward-open, and occluded states. Complemented by mutagene... More
