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Determination of optimum levels of binding antibody units (BAU) of new quantitative chemiluminescent immuno-assay (CLIA) in COVID-19 vaccinated volunteer blood donors

Transfus Apher Sci. 2024-04; 
Aseem Kumar Tiwari, Geet Aggarwal, Pratibha Kale, Neeti Yadav, Virendra Kumar, Gargi Singh, K Cheirmaraj, K Krishnan
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Proteins, Expression, Isolation and Analysis … Roche-S with GenScript sVNT assay. All these immunoassays had spike protein as its target … all three immunoassays when compared with GenScript cPass sVNT assay for neutralizing … Get A Quote

摘要

background: For assessment of COVID-19 vaccine efficacy, neutralization activity of anti-SARS-CoV-2 antibody is measured. This study was undertaken to determine optimum levels of binding antibody units (BAU/ml) in new quantitative chemiluminescent assay (CLIA) that corresponded to neutralizing potential (30% inhibition) of sVNT assay. methods: Ninety-one blood samples were analyzed by CLIA and sVNT assays. Test samples (n?=?75) were collected from blood donors post-2nd vaccination dose, while control samples (n?=?16) were archived pre-COVID donor samples. Correlation between CLIA and sVNT was calculated and receiver operating characteristic (ROC) curve was drawn and analyzed. results: Results indicated exce... More

關鍵詞

IgG antibodies, Immunoassay, Neutralization, Quantitative CLIA, SVNT
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