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Expanding the genetic toolbox for the obligate human pathogen

Front Bioeng Biotechnol. 2024-06; 
Nina Lautenschl?ger, Katja Schmidt, Carolin Schiffer, Thomas F Wulff, Karin Hahnke, Knut Finstermeier, Mo?se Mansour, Alexander K W Elsholz, Emmanuelle Charpentier
Products/Services Used Details Operation
Gene Synthesis … pyogenes, we have developed a comprehensive genetic … plasmids for gene expression and genetic manipulation, … obtained by gene synthesis (service provided by GenScript). … Get A Quote

摘要

Genetic tools form the basis for the study of molecular mechanisms. Despite many recent advances in the field of genetic engineering in bacteria, genetic toolsets remain scarce for non-model organisms, such as the obligatory human pathogen To overcome this limitation and enable the straightforward investigation of gene functions in , we have developed a comprehensive genetic toolset. By adapting and combining different tools previously applied in other Gram-positive bacteria, we have created new replicative and integrative plasmids for gene expression and genetic manipulation, constitutive and inducible promoters as well as fluorescence reporters for . The new replicative plasmids feature low- and high-copy re... More

關鍵詞

Streptococcus pyogenes, genetic engineering, inducible promoter, plasmid collection, reporter gene, scarless gene deletion, synthetic biology
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