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| Codon Optimization | After separation via SDS-PAGE (Genscript, China) and transferring to the?PVDF?membrane (Merck, USA), primary antibodies against TET2 (1:1000, Abcam, USA), FOXP3 (1:1000, Abcam, USA), RORγT (1:1000, Abcam, USA),?NLRP3?(1:1000, Abcam, USA), IL-1β (1:1000, Abcam, USA), cleaved?caspase 1?(1:1000, CST, USA), GSDMD-N (1:1000, Abcam, USA), TNF-α (1:1000, Abcam, USA), p-IκBα (1:1000, Abcam, USA), IκBα (1:1000, USA), p-P65 (1:1000, Abcam, USA), IκBα (1:1000, Abcam, USA), GAPDH (1:5000) and β-actin (1:5000) (Abcam, USA) were administrated at 4°C for 12 h.? | Get A Quote |
Introduction and objectives: MicroRNA-326 is abnormally expressed in autoimmune diseases, but its roles in autoimmune hepatitis (AIH) are unknown. In this study, we aimed to investigate the effect of miR-326 on AIH and the underlying mechanism.Materials and methods: Concanavalin A was administrated to induce AIH in mice and the expression levels of miR-326 and TET2 was evaluated by qRT-PCR and western blot, respectively. The percentages of Th17 and Treg cells were evaluated by flow cytometry and their marker proteins were determined by western blot and ELISA. The mitochondrial membrane potential (MMP) and ROS level were tested with the JC-1 kit and DCFH-DA assay. The binding relationships between miR-326 and TE... More
