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A Gamma-adapted subunit vaccine induces broadly neutralizing antibodies against SARS-CoV-2 variants and protects mice from infection

Nat Commun. 2024-02; 
Lorena M Coria , Juan Manuel Rodriguez , Agostina Demaria , Laura A Bruno , Mayra Rios Medrano , Celeste Pueblas Castro , Eliana F Castro , Sabrina A Del Priore , Andres C Hernando Insua , Ingrid G Kaufmann , Lucas M Saposnik , William B Stone , Lineia Prado , Ulises S Notaro , Ayelen N Amweg , Pablo U Diaz , Martin Avaro , Hugo Ortega , Ana Ceballos , Valeria Krum , Francisco M Zurvarra , Johanna E Sidabra , Ignacio Drehe , Jonathan A Baqué , Mariana Li Causi , Analia V De Nichilo , Cristian J Payes , Teresa Southard , Julio C Vega , Albert J Auguste , Diego E álvarez , Juan M Flo , Karina A Pasquevich , Juliana Cassataro
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Codon Optimization … The constructs were codon-optimized for CHO cell expression and synthesized by GenScript (Hong Kong Limited, Japan). All constructs were cloned into the pJC3UMCS-4 expression … Get A Quote

摘要

In the context of continuous emergence of SARS-CoV-2 variants of concern (VOCs), one strategy to prevent the severe outcomes of COVID-19 is developing safe and effective broad-spectrum vaccines. Here, we present preclinical studies of a RBD vaccine derived from the Gamma SARS-CoV-2 variant adjuvanted with Alum. The Gamma-adapted RBD vaccine is more immunogenic than the Ancestral RBD vaccine in terms of inducing broader neutralizing antibodies. The Gamma RBD presents more immunogenic B-cell restricted epitopes and induces a higher proportion of specific-B cells and plasmablasts than the Ancestral RBD version. The Gamma-adapted vaccine induces antigen specific T cell immune responses and confers protection agains... More

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