The gene family plays roles in biotic and abiotic stress, secondary metabolism, and other aspects in plants. They have been reported in , , , and other plants, but their roles in have not been determined. In this study, we analyzed the genome-wide identification and expression profile of the gene family in . A total of 51 members of the gene family were identified based on the genome and numbered in order from to . Their phylogenetic relationships, -elements in promoter region, gene structures and motif compositions, physicochemical properties, chromosome locations, and expression profiles, were analyzed. The results showed that the 51 were unevenly distributed on 10 chromosomes and could be clustered into 11 subfamilies. Furthermore, located in the same subfamilies presented similar intron/exon organization and motif composition. The expression levels of s in various tissues (flowers, bracts, vegetative leaves, stems, and seeds) were determined using reverse transcription quantitative polymerase chain reaction. The expression levels of s were higher in flowers and bracts. The 51 s were explored, and their structure, evolution, and expression pattern in different tissues of were characterized synthetically. The findings indicated that are essential for the growth and development of . These results provide a theoretical basis for subsequent research on hemp bZIP transcription factors and the cultivation of high-cannabidiol and low-tetrahydrocannabinol high-quality cannabis varieties.