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targeting of lentiviral vectors pseudotyped with the Tupaia paramyxovirus H glycoprotein bearing a cell-specific ligand

Mol Ther Methods Clin Dev. 2021-04; 
Takele Argaw, Michael P Marino, Andrew Timmons, Lindsey Eldridge, Kazuyo Takeda, Pingjuan Li, Anna Kwilas, Wu Ou, Jakob Reiser
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Codon Optimization Synthetic, codon-optimized versions of the TPMV H and F protein coding regions (GenBank:?AF079780.2) were prepared by GenScript (Piscataway, NJ, USA) Get A Quote

摘要

Despite their exceptional capacity for transgene delivery , lentiviral (LV) vectors have been slow to demonstrate clinical utility in the context of applications. Unresolved safety concerns related to broad LV vector tropism have limited LV vectors to applications. Here, we report on a novel LV vector-pseudotyping strategy involving envelope glycoproteins of Tupaia paramyxovirus (TPMV) engineered to specifically target human cell-surface receptors. LV vectors pseudotyped with the TPMV hemagglutinin (H) protein bearing the interleukin (IL)-13 ligand in concert with the TPMV fusion (F) protein allowed efficient transduction of cells expressing the human IL-13 receptor alpha 2 (IL-13Rα2). Immunodeficient mice b... More

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