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A scalable serology solution for profiling humoral immune responses to SARS-CoV-2 infection and vaccination

Clin Transl Immunology. 2022-03; 
Karen Colwill, Yannick Galipeau, Matthew Stuible, Christian Gervais, Corey Arnold, Bhavisha Rathod, Kento T Abe, Jenny H Wang, Adrian Pasculescu, Mariam Maltseva, Lynda Rocheleau, Martin Pelchat, Mahya Fazel-Zarandi, Mariam Iskilova, Miriam Barrios-Rodiles, Linda Bennett, Kevin Yau, Fran?ois Cholette, Christine Mesa, Angel X Li, Aimee Paterson, Michelle A Hladunewich, Pamela J Goodwin, Jeffrey L Wrana, Steven J Drews, Samira Mubareka, Allison J McGeer, John Kim, Marc-André Langlois, Anne-Claude Gingras, Yves Durocher
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Codon Optimization … by a commercial anti-N antibody to quantify the anti-N response (see Methods). As described … VHH and mAb sequences were synthesised by GenScript using C. griseus codon bias and … Get A Quote

摘要

unassigned: Antibody testing against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has been instrumental in detecting previous exposures and analyzing vaccine-elicited immune responses. Here, we describe a scalable solution to detect and quantify SARS-CoV-2 antibodies, discriminate between natural infection- and vaccination-induced responses, and assess antibody-mediated inhibition of the spike-angiotensin converting enzyme 2 (ACE2) interaction. unassigned: We developed methods and reagents to detect SARS-CoV-2 antibodies by enzyme-linked immunosorbent assay (ELISA). The main assays focus on the parallel detection of immunoglobulin (Ig)Gs against the spike trimer, its receptor binding domain (RBD... More

關(guān)鍵詞

SARS‐CoV‐2, antibody detection, antibody neutralisation, assay development and standardisation, high‐throughput screening
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