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SARS-CoV-2 S1 NanoBiT: A nanoluciferase complementation-based biosensor to rapidly probe SARS-CoV-2 receptor recognition

Biosens Bioelectron. 2021-03; 
Taha Azad, Ragunath Singaravelu, Emily E F Brown, Zaid Taha, Reza Rezaei, Rozanne Arulanandam, Stephen Boulton, Jean-Simon Diallo, Carolina S Ilkow, John C Bell
Products/Services Used Details Operation
Codon Optimization … 2.2. Plasmids. Codon optimized coding sequences for S1 and ACE2 ectomain were ordered from GenScript (Piscataway, NJ, USA). Sequences are shown in Table S1. Bacterial expression plasmid (pSb_init) encoding synthetic?… Get A Quote

摘要

As the COVID-19 pandemic continues, there is an imminent need for rapid diagnostic tools and effective antivirals targeting SARS-CoV-2. We have developed a novel bioluminescence-based biosensor to probe a key host-virus interaction during viral entry: the binding of SARS-CoV-2 viral spike (S) protein to its receptor, angiotensin-converting enzyme 2 (ACE2). Derived from Nanoluciferase binary technology (NanoBiT), the biosensor is composed of Nanoluciferase split into two complementary subunits, Large BiT and Small BiT, fused to the Spike S1 domain of the SARS-CoV-2 S protein and ACE2 ectodomain, respectively. The ACE2-S1 interaction results in reassembly of functional Nanoluciferase, which catalyzes a biolumines... More

關鍵詞

ACE2, COVID-19, Luminescent biosensor, Receptor interaction, SARS-CoV2, Spike
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