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The Alphaviral Capsid Protein Inhibits IRAK1-Dependent TLR Signaling

Viruses. 2021-02; 
V Douglas Landers, Daniel W Wilkey, Michael L Merchant, Thomas C Mitchell, Kevin J Sokoloski
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Gene Synthesis … All DNA fragments for the generation of the clones above were synthesized by Genscript (GenScript USA Inc., Piscataway, NJ, USA) and assembled using the Gibson Assembly mastermix available from Synthetic Genomics, Inc?… Get A Quote

摘要

Alphaviruses are arthropod-borne RNA viruses which can cause either mild to severe febrile arthritis which may persist for months, or encephalitis which can lead to death or lifelong cognitive impairments. The non-assembly molecular role(s), functions, and protein-protein interactions of the alphavirus capsid proteins have been largely overlooked. Here we detail the use of a BioID2 biotin ligase system to identify the protein-protein interactions of the Sindbis virus capsid protein. These efforts led to the discovery of a series of novel host-pathogen interactions, including the identification of an interaction between the alphaviral capsid protein and the host IRAK1 protein. Importantly, this capsid-IRAK1 inte... More

關(guān)鍵詞

IRAK1, alphavirus, capsid, toll like receptors (TLR)
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