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Cutting Edge: Mouse SARS-CoV-2 Epitope Reveals Infection and Vaccine-Elicited CD8 T Cell Responses

J Immunol. 2021-01; 
Vineet Joag, Sathi Wijeyesinghe, J Michael Stolley, Clare F Quarnstrom, Thamotharampillai Dileepan, Andrew G Soerens, Jules A Sangala, Stephen D O'Flanagan, Noah V Gavil, Sung-Wook Hong, Siddheshvar Bhela, Sailaja Gangadhara, Eyob Weyu, William E Matchett, Joshua Thiede, Venkatramana Krishna, Maxim C-J Cheeran, Tyler D Bold, Rama Amara, Peter Southern, Geoffrey T Hart, Luca Schifanella, Vaiva Vezys, Marc K Jenkins, Ryan A Langlois, David Masopust
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Gene Synthesis … http://www.jimmunol.org/ Downloaded from Page 3. optimization tool (GenScript). A 1352-bp gene block consisting of SARS- CoV-2 N together with ~15-bp overlaps at the 59 and 39 ends for infusion cloning was synthesized (GenScript). pVAX1 Vector (catalog no?… Get A Quote

摘要

The magnitude of SARS-CoV-2-specific T cell responses correlates inversely with human disease severity, suggesting T cell involvement in primary control. Whereas many COVID-19 vaccines focus on establishing humoral immunity to viral spike protein, vaccine-elicited T cell immunity may bolster durable protection or cross-reactivity with viral variants. To better enable mechanistic and vaccination studies in mice, we identified a dominant CD8 T cell SARS-CoV-2 nucleoprotein epitope. Infection of human ACE2 transgenic mice with SARS-CoV-2 elicited robust responses to H2-D/N, and 40% of HLA-A*02 COVID-19 PBMC samples isolated from hospitalized patients responded to this peptide in culture. In mice, i.m. prime-boost ... More

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