Gag p24 is a Marker of HIV Expression in Tissues and Correlates with Immune Response
| Products/Services Used |
Details |
Operation |
| Proteins, Expression, Isolation and Analysis |
… Merck & Co., Inc. 770 Sumneytown Pike, Mailstop: 14-3212, West Point, PA, 19486?… 280
streptavidin beads (ThermoFisher Scientific) containing 10 ug/ml mouse IgG (GenScript). Following
3h rocking at 4°C, lysates were centrifuged and supernatants run on HD-1 Analyzer [6]?… |
Get A Quote |
We demonstrate that HIV gag p24 protein is more readily detected in gut and lymph node tissues than in blood CD4+ T-cells and correlates better with CD4 count during antiretroviral therapy (ART). Gut p24 levels also measurably decline with ART in natural controllers. During ART, gut p24 expression is more strongly associated both with HIV-specific CD8+ T-cell frequency and plasma sCD14 levels than gut HIV RNA expression. This study supports using gag p24 as a marker of HIV expression in HIV+ tissues to study effects of viral persistence and to monitor efficacy of treatment in HIV-based clearance studies.
We demonstrate that HIV gag p24 protein is more readily detected in gut and lymph node tissues than in blood CD4+ T-cells and correlates better with CD4 count during antiretroviral therapy (ART). Gut p24 levels also measurably decline with ART in natural controllers. During ART, gut p24 expression is more strongly associated both with HIV-specific CD8+ T-cell frequency and plasma sCD14 levels than gut HIV RNA expression. This study supports using gag p24 as a marker of HIV expression in HIV+ tissues to study effects of viral persistence and to monitor efficacy of treatment in HIV-based clearance studies.
Gag p24, HIV, HIV persistence, biomarker, fine needle aspirates, lymph nodes, rectal biopsy
