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A flow cytometry-based assay for serological detection of anti-spike antibodies in COVID-19 patients

STAR Protoc. 2021-06;

Yun Shan Goh, Lisa F P Ng, Laurent Renia

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摘要

One of the key public health strategies in coronavirus 2019 disease (COVID-19) management is the early detection of infected individuals to limit the transmission. As a result, serological assays have been developed to complement PCR-based assays. Here, we report the development of a flow cytometry-based assay to detect antibodies against full-length SARS-CoV-2 Spike protein (S protein) in COVID-19 patients. The assay is time-efficient and sensitive, being able to capture the wider repertoire of antibodies against the S protein.