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Production of Protocatechuic Acid from -Hydroxyphenyl (H) Units and Related Aromatic Compounds Using an Aspergillus niger Cell Factory

MBio. 2021-06; 
Ronnie J M Lubbers, Ronald P de Vries
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Gene Synthesis … phyA gene was synthesized based on the reference sequence (NRRL3_4569) in pET23b containing a C-terminal hexahistidine tag (GenScript Biotech, Leiden?… FF 1-ml column coupled with the ?KTA start system (GE Healthcare Life Sciences, Uppsala, Sweden) using the setup?… Get A Quote

摘要

Protocatechuic acid (3,4-dihydroxybenzoic acid) is a chemical building block for polymers and plastics. In addition, protocatechuic acid has many properties of great pharmaceutical interest. Much research has been performed in creating bacterial protocatechuic acid production strains, but no protocatechuic acid-producing fungal cell factories have been described. The filamentous fungus Aspergillus niger can produce protocatechuic acid as an intermediate of the benzoic acid metabolic pathway. Recently, the -hydroxybenzoate--hydroxylase () and protocatechuate 3,4-dioxygenase () of A. niger have been identified. It has been shown that the deletion mutant is still able to grow on protocatechuic acid. This led to t... More

關鍵詞

3,4-dihydroxybenzoic acid, Aspergillus niger, benzoic acid metabolic pathway, caffeic acid, filamentous fungi, fungi, p-coumaric acid, protocatechuate hydroxylase
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