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Paper Title "Hu7CG2: A Novel Humanized Anti-Epidermal Growth Factor Receptor (EGFR) Biparatopic Nanobody"

Mol Biotechnol. 2021-03; 
Jafar Sharifi, Mohammad Reza Khirehgesh, Bahman Akbari, Bijan Soleymani, Kamran Mansouri
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Codon Optimization … The hu7CG2 sequence was codon-optimized to E. coli codon usage by http://www. genscript.com/cgi-bin/tools/rare. For rare codon analysis, the sequence was analyzed by an online https://www.genscript.com/tools/rare-codon-analysis tool?… Get A Quote

摘要

Targeted therapy is an effective and appropriate approach with low side effects in cancer therapy compared with other treatment approaches. Epidermal growth factor receptor, EGFR, is a favorable biomarker as targeted therapy because it overexpresses in several cancers. Monoclonal antibodies are common agents for targeted therapy. Nanobody is the smallest format of monoclonal antibodies with unique properties that include hiding epitope targeting, high stability, low production cost, and ease of connection to other components. The main challenge in targeted therapy by monoclonal antibodies is their immunogenicity due to their non-human nature. In this study, we designed, constructed, and evaluated a novel humani... More

關鍵詞

Biparatope, EGFR, Hu7CG2, Humanized, Nanobody, Targeted therapy
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