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Collaboration between the Fab and Fc contribute to maximal protection against SARS-CoV-2 following NVX-CoV2373 subunit vaccine with Matrix-M? vaccination

Res Sq. 2021-02; 
Galit Alter, Matthew Gorman, Nita Patel, Mimi Guebre-Xabier, Alex Zhu, Caroline Atyeo, Krista Pullen, Carolin Loos, Yenny Goez-Gazi, Ricardo Carrion, Jing-Hui Tian, Dansu Yuan, Kathryn Bowman, Bin Zhou, Sonia Maciejewski, Marisa McGrath, James Logue, Matthew Frieman, David Montefiori, Sharon Schendel, Erica Ollmann Saphire, Douglas Lauffenburger, Ann Greene, Alyse Portnoff, Michael Massare, Larry Ellingsworth, Gregory Glenn, Gale Smith, Colin Mann, Fatima Amanat, Florian Krammer
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Gene Synthesis … The synthetic transgene was codon optimized and engineered into the baculovirus vector (BV2373) for expression in Spodoptera frugiperda (Sf9) insect cells (GenScript, Piscataway, NJ, USA). NVX-CoV2373 spike trimers were detergent extracted from the plasma?… Get A Quote

摘要

Recently approved vaccines have already shown remarkable protection in limiting SARS-CoV-2 associated disease. However, immunologic mechanism(s) of protection, as well as how boosting alters immunity to wildtype and newly emerging strains, remain incompletely understood. Here we deeply profiled the humoral immune response in a cohort of non-human primates immunized with a stable recombinant full-length SARS-CoV-2 spike (S) glycoprotein (NVX-CoV2373) at two dose levels, administered as a single or two-dose regimen with a saponin-based adjuvant Matrix-M?. While antigen dose had some effect on Fc-effector profiles, both antigen dose and boosting significantly altered overall titers, neutralization and Fc-effecto... More

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