| Products/Services Used | Details | Operation |
|---|---|---|
| Proteins, Expression, Isolation and Analysis | … Anti-myc antibody was purchased from Proteintech (Wuhan, China). Protein L beads for domain antibody purification were purchased from Genscript Co. Ltd. (Nanjing, China). Nonfat milk powder was purchased from Sangon Biotech (Shanghai, … | Get A Quote |
Heparin-binding epidermal growth factor (EGF)-like growth factor (HB-EGF), which binds to the EGF receptor, plays an important role in the occurrence and development of inflammation in various diseases. HB-EGF mediates the progression of ovarian cancer and is associated with disease prognosis. Thus, a specific humanized antibody to HB-EGF with high affinity is important. In this study, a humanized domain antibody (VH) against HB-EGF was discovered through phage display technology. The domain antibody was expressed in HB2151 cells and purified from the supernatant using protein L, and were used to test the its effect in invasion and migration of ovarian cancer SKOV3. A domain antibody against HB-EGF was discov... More
