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Gene Synthesis> | … Harbor, NY, 1989). Further, nucleic acid constructs according to the present invention may be synthesized without template, and may be obtained from various commercial suppliers (eg Genscript Corporation). The nucleic acid … | Get A Quote |
objective: Endometriosis is a disease characterized by ectopic implantation of endometrium and impaired immune responses. To explore its pathogenic mechanisms, we studied the local and systemic cytokine mRNA profiles and their role in the immunity of patients with endometriosis and healthy controls. methods: mRNA for eleven cytokines defining cytotoxic Th1, humoral Th2, regulatory Tr1/Th3 and inflammatory cytokine profiles were characterized locally in endometriotic tissue and endometrium, and systemically in PBMCs from women with endometriosis and healthy controls, using real-time qRT-PCR. In addition, immunohistochemical stainings with monoclonal antibodies were performed looking for T regulatory cells in end... More
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