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Characterization of species of Fusarium causing root rot of Soybean (Glycine max L) in South Dakota, USA

Canadian Journal of Plant Pathology. 2020-03; 
Paul N. Okello, Kristina Petrovic, Asheesh K. Singh, Brian Kontz & Febina M. Mathew
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Gene Synthesis … concentration of 0.25× TBE buffer to confirm amplification. DNA samples of the 57 isolates were sequenced (GenScript USA Inc., Piscataway, NJ) using the same primers. The DNA sequences were subjected to BLASTN searches … Get A Quote

摘要

In 2014, 2000 plants showing root rot symptoms were collected from 200 commercial soybean (Glycine max L.) fields in South Dakota, USA. One thousand one hundred thirty fungal isolates obtained from the samples were identified as Fusarium species by morphology and sequencing of the translation elongation factor 1-alpha gene. Fifty-one percent were F. graminearum, 30.0% were F. acuminatum, 8.0% belonged to the F. oxysporum species complex, 3.4% were F. armeniacum, 2.0% were F. proliferatum, 1.6% were F. virguliforme, 1.2% belonged to the F. solani species complex, 0.4% were F. nanum (syn. F. incarnatum-equiseti species complex), 0.1% were F. bulbicola (syn. F. fujikuroi species complex) and 0.1% were F. commune. ... More

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