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Characterization of a cdc14 null allele in Drosophila melanogaster

Biol Open. 2018;

Neitzel LR, Broadus MR, Zhang N, Sawyer L, Wallace HA, , Merkle JA, Jodoin JN, Sitaram P, Crispi EE, Rork W, Lee LA0, Pan D, Gould KL, Page-McCaw A, , Lee E,

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Proteins, Expression, Isolation and Analysis	For late third instar larvae, pools of ten were collected and homogenized in 200 μl of running buffer (Fig. 1F, S2B,C), and protein equivalent to one-half of a larva (10 μl) was used. All samples were run on ExpressPlustm PAGE gels 4-20% from GenScript.	Get A Quote

摘要

Cdc14 is an evolutionarily conserved serine/threonine phosphatase. Originally identified in Saccharomyces cerevisiae as a cell cycle regulator, its role in other eukaryotic organisms remains unclear. In Drosophila melanogaster, Cdc14 is encoded by a single gene, thus facilitating its study. We found that Cdc14 expression is highest in the testis of adult flies and that cdc14 null flies are viable. cdc14 null female and male flies do not display altered fertility. cdc14 null males, however, exhibit decreased sperm competitiveness. Previous studies have shown that Cdc14 plays a role in ciliogenesis during zebrafish development. In Drosophila, sensory neurons are ciliated. We found that the Drosophila cdc14 null m... More