| Products/Services Used | Details | Operation |
|---|---|---|
| Gene Synthesis | The primer set 341F with GC clamp and 534R was used to selectively amplify the V3 region of 16S rRNA gene from the total DNA [19]. One μL from each total DNA sample was mixed with 10 pmol of each primer, 0.25 mM of dNTP, 5 μL of 10x Green?Taq?PCR buffer, and 1 μL of Green?Taq?DNA polymerase to make a 50 μL PCR mixture (GenScript, Piscataway, NJ). PCR was set for initial denaturation at 95 °C for 5 minutes, followed by thirty five cycles, each comprising 20 seconds at 95 °C, 45 seconds at 55 °C, and 45 seconds at 72 °C. The reactions were finished with a final extension at 72 °C for 7 minutes. The amplification products were checked for integrity using 1.5% agarose gel electrophoresis. | Get A Quote |
The persistence and recurrence of tar balls causes detrimental effects on coastal wildlife, tourism, and fisheries. Previously, the human pathogen?Vibrio vulnificus?was detected in marine tar balls in the Gulf of Mexico. Marine tar balls also migrate to low-salt water bodies near the shore. The bacterial communities in tar balls from these environments, however, have not been fully characterized. Herein we describe our studies on the effect of reduced salinity on bacterial communities in marine tar balls. Tar balls collected from the Gulf of Mexico were incubated in deionized water for six months, and their microbial fingerprints were visualized using denaturing gradient gel electrophoresis. Our data show tha... More
