| Products/Services Used | Details | Operation |
|---|---|---|
| Gene Synthesis | The specificity primer pairs used in this study are listed in?Table 2. The?L. monocytogenes?specific primers were designed to amplify a 268?bp segment from the?hly?gene?[31]?that was used to encoding the?virulence factor?listeriolysin O. The sequences designed to amplify 475?bp segment from?16S?rRNA?gene?[32]. The?hlyprimers were designed using Oligo 6.0 software (http://www.oligo.net/). The specificity of the sequences was tested by searched against NCBI’s non-redundant database using the BLASTN algorithm?[33]. All primers were synthesized by GenScript (Nanjing) Co., Ltd. (Nanjing, China). | Get A Quote |
In this study,?vancomycin?(Van) functionalized PEGylated-magnetic?nanoparticles(Van-PMs) combined with?polymerase chain reaction?(PCR) showed great potential for highly efficient detection of?L. monocytogenes?at low infectious dose. Herein, Van-PMs played as universal?molecular probes?for targeting?bacterial cells, while PEG was used to help Van close to D-alanyl-d-alanine (D-Ala-d-Ala) moieties on surface of?L. monocytogenes. Under optimum conditions, the developed Van-PMs complexes exhibited extremely?high capture efficiency?(CE) in a 10?mL bacterium suspension with the value was higher than 90% and 83% in the?phosphate-buffered saline?(PBS) solution and lettuce samples, respectively. Meanwhil... More
