Intramuscular?(IM)?immunization?is generally considered incapable of generating a protective mucosal immune response. In the swine industry, attempts to develop a safe and protective vaccine for controlling?porcine?epidemic?diarrhea?(PED) via an IM route of administration have been unsuccessful. In the present study,?porcine?chemokine ligand proteins CCL25, 27, and 28 were constructed and stably expressed in the mammalian expression system. IM co-administration of inactivated PEDV (iPEDV) particles with different CC chemokines and Freund's adjuvants resulted in recruiting CCR9+ and/or CCR10+ inflammatory cells to the injection site, thereby inducing superior systemic PEDV specific IgG, fecal IgA, and viral neutralizing antibodies in?pigs. Moreover,?pigs?immunized with iPEDV in combination with CCL25 and CCL28 elicited?substantial?protection?against a virulent PEDV challenge. We show that the?porcine?CC chemokines could be novel adjuvants for developing IM vaccines for modulating mucosal immune responses against mucosal transmissible pathogens in?pigs.