Autismspectrum?disorder?(ASD) is a complex and multifactorial heterogeneous?disorder. Previous investigations have revealed the association between the immune system and ASD, which is characterized by impaired communication skills.?Inflammatory?response through?CD45?cells?plays a?key?role in the pathogenesis of several autoimmune disorders; however, the molecular mechanism of?CD45?cells?in ASD is not clearly defined.In this study, we investigated the role of?CD45?signaling in children with ASD. In this study, we aimed to investigate the possible?involvement?of?CD45?cells?expressing?granulocyte-macrophage?colony-stimulating?factor?and?inflammatory?transcription?factors?in ASD. Flow cy... More
Autismspectrum?disorder?(ASD) is a complex and multifactorial heterogeneous?disorder. Previous investigations have revealed the association between the immune system and ASD, which is characterized by impaired communication skills.?Inflammatory?response through?CD45?cells?plays a?key?role in the pathogenesis of several autoimmune disorders; however, the molecular mechanism of?CD45?cells?in ASD is not clearly defined.In this study, we investigated the role of?CD45?signaling in children with ASD. In this study, we aimed to investigate the possible?involvement?of?CD45?cells?expressing?granulocyte-macrophage?colony-stimulating?factor?and?inflammatory?transcription?factors?in ASD. Flow cytometric analysis, using peripheral blood mononuclear?cells?(PBMC), revealed the numbers of GM-CSF-, IFN-γ-, IL-6-, IL-9-, IL-22-, T-bet-, pStat3-, Helios-, and Stat6-producing?CD45+?cells?in children with ASD and children in the control group. We further evaluated the mRNA and protein expression levels of GM-CSF in PBMC by RT-PCR and western blotting analysis. Our results revealed that the children with ASD exhibited significantly higher numbers of?CD45+GM-CSF+,?CD45+IFN-γ+,?CD45+IL-6+,?CD45+IL-9+,?CD45+IL-22+,?CD45+T-bet+, and?CD45+pStat3+?cells?compared with the control group. We also found that the children with ASD showed a lower number of?CD45+Helios+and?CD45+Stat6+?cells?compared with the control group. Furthermore, the children with ASD showed higher GM-CSF mRNA and protein expression levels compared with the control group. These results indicated that?CD45?could play an essential role in the immune abnormalities of ASD. Further investigation of the role of?CD45?in neurodevelopment in ASD is warranted.
