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Genetic Determinants of CYP2C19 Gene* 2 and* 3 Loss of Function Alleles and Response to Anti Platelet Therapy (Clopidogrel) and Cardiovascular Events(A

Biology and Medicine. 2015; 
Irfan Ahmad Bhat , Arshad A. Pandith , Irfan Yaqoob, Shehjar Faheem, Imran Hafeez, Jahangir R Beig, Zafar A. Shah and Khurshed Iqbal
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Molecular Biology Tools … PCR was carried out in a final volume of 25 uL containing 50 ng genomic DNA template, 1x PCR buffer (Biotools, B and M Labs, Madrid, Spain) with 2 mmol/L MgCl2, 04 mmol/L of each primer (Genscript, Piscataway, NJ), 50 mmol/L dNTPs (Biotools, B and M Labs), and 05 U … Get A Quote

摘要

Background: Studies have demonstrated that the mutant *2 and*3 allele of the CYP2C19 loss-of-function polymorphism is associated with diminished metabolization of clopidogrel and an attenuated platelet response to clopidogrel treatment. Since no such study has been conducted in this region, we examined CYP2C19 polymorphism in Acute Coronary Syndrome (ACS) patients on clopidogrel treatment, and its effect on the cardiovascular outcomes. Material and Methods: A total of 100 samples of ACS were included in this study and genotyping of CYP2C19 *2 and *3 gene polymorphisms was performed by a Polymerase chain reaction-Restriction fragment length polymorphism (PCR-RFLP). Results: The distribution of CYP2C19*2 allele w... More

關鍵詞

Acute Coronary syndrome; CYP2C19; Clopidogrel; Poormetabolizer; Allele
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