国产精品久久久久久永久牛牛,55国产福利在线视频,成人午夜精品视频在线观看

至今,GenScript的服務及產(chǎn)品已被Cell, Nature, Science, PNAS等1300多家生物醫(yī)藥類雜志引用近萬次,處于行業(yè)領先水平。NIH、哈佛、耶魯、斯坦福、普林斯頓、杜克大學等約400家全球著名機構使用GenScript的基因合成、多肽服務、抗體服務和蛋白服務等成功地發(fā)表科研成果,再次證明GenScript 有能力幫助業(yè)內(nèi)科學家Make research easy.

Efficient generation of mouse models of human diseases via ABE- and BE-mediated base editing.

Nat Commun. 2018; 
LiuZhen,LuZongyang,YangGuang,HuangShisheng,LiGuanglei,F(xiàn)engSongjie,LiuYajing,LiJianan,YuWenxia,ZhangYu,ChenJia,SunQiang,HuangXi
Products/Services Used Details Operation
Gene Synthesis The lysed and neutralized sample was added with 5 μl of a 400 μM solution of random primers (Genscript, Nanjing, China), 6 μl of 10× PCR buffer (Takara, Dalian, China), 3 μl of a mixture of the 4 dNTPs (each at 2.5 mM), and 1 μl of Taq polymerase (Takara, Dalian, China) and brought to 60 μl with water. Get A Quote

摘要

A recently developed adenine base editor (ABE) efficiently converts A to G and is potentially useful for clinical applications. However, its precision and efficiency in vivo remains to be addressed. Here we achieve A-to-G conversion in vivo at frequencies up to 100% by microinjection of ABE mRNA together with sgRNAs. We then generate mouse models harboring clinically relevant mutations at Ar and Hoxd13, which recapitulates respective clinical defects. Furthermore, we achieve both C-to-T and A-to-G base editing by using a combination of ABE and SaBE3, thus creating mouse model harboring multiple mutations. We also demonstrate the specificity of ABE by deep sequencing and whole-genome sequencing (WGS). Ta... More

關鍵詞

              主站蜘蛛池模板: 泗洪县| 漳浦县| 吉首市| 富裕县| 太仓市| 芜湖县| 平江县| 普陀区| 岫岩| 皋兰县| 鱼台县| 西乡县| 濮阳市| 南华县| 偃师市| 山东省| 平安县| 柳州市| 仁化县| 浠水县| 静海县| 来凤县| 汉川市| 南陵县| 贞丰县| 朝阳区| 金平| 碌曲县| 荆门市| 绩溪县| 都江堰市| 青神县| 思茅市| 东海县| 清水河县| 仙桃市| 来安县| 瑞金市| 岳西县| 乌拉特中旗| 图片|