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Codon Optimization> | … report (Mutalik et al., 2013). pEEK2 has been made available through AddGene (AddGene number: 83492). All the heterogenous genes were codon optimized and synthesized by GenScript. All the endogenous genes were … | Get A Quote |
Isobutanol is a flammable compound that can be used as a biofuel due to its high energy density and suitable physical and chemical properties. In this study, we examined the capacity of engineered strains of PCC 6803 containing the α-ketoisovalerate decarboxylase from and different heterologous and endogenous alcohol dehydrogenases (ADH) for isobutanol production. A strain expressing an introduced without any additional copy of ADH produced 3?mg?L OD isobutanol in 6 days. After the cultures were supplemented with external addition of isobutyraldehyde, the substrate for ADH, 60.8?mg?L isobutanol was produced after 24?h when OD was 0.8. The in vivo activities of four different ADHs, two heterolog... More