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Effect of zinc intake on hepatic autophagy during acute alcohol intoxication.

Biometals. 2018-04; 
LiuzziJuan P,NarayananVijaya,DoanHuong,YooChan
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Proteins, Expression, Isolation and Analysis … Cellular protein content was determined with a micro BCA protein assay kit (Pierce, Rockford, IL). Aliquots of homogenates were diluted with an equal volume of 5 × Laemmli sample buffer and resolved on 8-16% polyacrylamide gels (Genscript, Piscataway, NJ) … Get A Quote

摘要

Autophagy is a conserved mechanism that plays a housekeeping role by eliminating protein aggregates and damaged organelles. Recent studies have demonstrated that acute ethanol intoxication induces hepatic autophagy in mice. The effect of dietary zinc intake on hepatic autophagic flux during ethanol intoxication has not been evaluated using animal models. Herein, we investigated whether zinc deficiency and excess can affect autophagic flux in the liver in mice and in human hepatoma cells acutely exposed to ethanol. A mouse model of binge ethanol feeding was utilized to analyze the effect of low, adequate, and high zinc intake on hepatic autophagic flux during ethanol intoxication. Autophagic flux was infer... More

關(guān)鍵詞

Autophagy,Ethanol,Liver,Mice,Zinc,Zinc in
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