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NpeA is a secreted Type IV effector containing an N-WASP-binding short linear motif that promotes niche formation

MBio. 2024-06; 
Agostina Giménez, Mariela G Del Giudice, Paula V López, Francisco Guaimas, Hugo Sámano-Sánchez, Toby J Gibson, Lucía B Chemes, Carlos O Arregui, Juan E Ugalde, Cecilia Czibener
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Gene Synthesis … The NpeA DNA fragment coding a region from amino acids 90–170 was synthesized (Genscript) and cloned in the vector pET32b (Novagen) in the BamHI and EcoRI sites. … Get A Quote

摘要

The modulation of actin polymerization is a common theme among microbial pathogens. Even though microorganisms show a wide repertoire of strategies to subvert the activity of actin, most of them converge in the ones that activate nucleating factors, such as the Arp2/3 complex. spp. are intracellular pathogens capable of establishing chronic infections in their hosts. The ability to subvert the host cell response is dependent on the capacity of the bacterium to attach, invade, avoid degradation in the phagocytic compartment, replicate in an endoplasmic reticulum-derived compartment and egress. Even though a significant number of mechanisms deployed by in these different phases have been identified and characte... More

關(guān)鍵詞

Brucella, N-WASP, Type IV effector, bab2_0195 gene
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