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Detection of avian reovirus (ARV) by ELISA based on recombinant σB, σC and σNS full-length proteins and protein fragments

J Med Microbiol. 2024-06; 
Tatiana Reichert Assun??o de Matos, Ana Paula Gori Palka, Claudemir de Souza, Stenio Perdig?o Fragoso, Daniela Parada Pavoni
Products/Services Used Details Operation
Gene Synthesis … from GenScript (New Jersey, USA) with sequences optimized for bacterial expression. The strain S1133 was chosen since it was used in the administered vaccines. The synthetic genes … Get A Quote

摘要

Avian reovirus (ARV) is associated with arthritis/tenosynovitis and malabsorption syndrome in chickens. The σC and σB proteins, both exposed to the virus capsid, are highly immunogenic and could form the basis for diagnostic devices designed to assess the immunological status of the flock. Commercial ARV ELISAs cannot distinguish between vaccinated and infected animals and might not detect circulating ARV strains. We aimed to develop a customized test to detect the circulating field ARV strains as well as distinguish between vaccinated and unvaccinated animals. We developed ELISA assays based on recombinant (r) σB, σC and the nonstructural protein σNS and tested them using antisera of vaccinated and unvacc... More

關(guān)鍵詞

ELISA, antigenic regions, avian reovirus, diagnosis
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