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Chimeric proteins of Mycoplasma hyopneumoniae as vaccine and preclinical model for immunological evaluation

Braz J Microbiol. 2024-01; 
Marcus Rebou?as Santos, Leonardo Teófilo Toledo, ênio José Bassi, Wagnner José Nascimento Porto, Gustavo Costa Bressan, Maria Aparecida S Moreira, Yung-Fu Chang, Abelardo Silva-Júnior
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Proteins, Expression, Isolation and Analysis … (Genscript, NJ, USA), and the N-terminus 6 × histidine-tagged chimeric proteins were … Protein purification was performed by affinity chromatography using a HisTrap crude FF (GE)… Get A Quote

摘要

Mycoplasma hyopneumoniae (M. hyopneumoniae) is a primary agent of porcine enzootic pneumonia, a disease that causes significant economic losses to pig farming worldwide. Commercial vaccines induce partial protection, evidencing the need for a new vaccine against M. hyopneumoniae. In our work, three chimeric proteins were constructed, composed of potentially immunogenic domains from M. hyopneumoniae proteins. We designed three chimeric proteins (Q1, Q2, and Q3) based on bioinformatics analysis that identified five potential proteins with immunogenic potential (MHP418, MHP372, MHP199, P97, and MHP0461). The chimeric proteins were inoculated in the murine model to evaluate the immune response. The mice vaccinated ... More

關鍵詞

Mycoplasma hyopneumoniae, Cytokines, Mouse model, Recombinant protein, Serology, Vaccine
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