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Glycan Profile and Sequence Variants of Certified Ricin Reference Material and Other Ricin Samples Yield Unique Molecular Signature Features

Toxins (Basel). 2024-05; 
Roland Josuran, Andreas Wenger, Christian Müller, Bettina Kampa, Sylvia Worbs, Brigitte G Dorner, Sabina Gerber
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Proteins, Expression, Isolation and Analysis … protein were performed as previously described. Briefly, the C-terminal LDP protein of LDM … LDP-SGGPEGGS-VL protein sequences were synthesized by GenScript company (Nanjing, … Get A Quote

摘要

A certified reference material of ricin (CRM-LS-1) was produced by the EuroBioTox consortium to standardise the analysis of this biotoxin. This study established the -glycan structures and proportions including their loci and occupancy of ricin CRM-LS-1. The glycan profile was compared with ricin from different preparations and other cultivars and isoforms. A total of 15 different oligomannosidic or paucimannosidic structures were identified in CRM-LS-1. Paucimannose was mainly found within the A-chain and oligomannose constituted the major glycan type of the B-chain. Furthermore, the novel primary structure variants E138 and D138 and four different C-termini of the A-chain as well as two B-chain variants V250 ... More

關鍵詞

N-glycosylation, cultivars, glycan profile, ricin, sequence variants
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