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YeeD is an essential partner for YeeE-mediated thiosulfate uptake in bacteria and regulates thiosulfate ion decomposition

PLoS Biol. 2024-04; 
Mai Ikei, Ryoji Miyazaki, Keigo Monden, Yusuke Naito, Azusa Takeuchi, Yutaro S Takahashi, Yoshiki Tanaka, Keina Murata, Takaharu Mori, Muneyoshi Ichikawa, Tomoya Tsukazaki
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Gene Synthesis … clearly defined, although 1 candidate gene is yeeD, which resides … coli yeeE gene, yeeD was shown to be involved in thios… by Gene Synthesis and Mutagenesis (SC1441, GenScript) or … Get A Quote

摘要

Uptake of thiosulfate ions as an inorganic sulfur source from the environment is important for bacterial sulfur assimilation. Recently, a selective thiosulfate uptake pathway involving a membrane protein YeeE (TsuA) in Escherichia coli was characterized. YeeE-like proteins are conserved in some bacteria, archaea, and eukaryotes. However, the precise function of YeeE, along with its potential partner protein in the thiosulfate ion uptake pathway, remained unclear. Here, we assessed selective thiosulfate transport via Spirochaeta thermophila YeeE in vitro and characterized E. coli YeeD (TsuB) as an adjacent and essential protein for YeeE-mediated thiosulfate uptake in vivo. We further showed that S. thermophila Y... More

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