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Preparation and immunogenicity evaluation of C-HapS-P6 fusion protein vaccine against nontypeable Haemophilus influenzae in mice

Int J Med Microbiol. 2024-03; 
Nan Hu, Weifeng Li, Zihong Zhao, Yueli Chang, Cai Wang, Yutuo Zhang
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Proteins, Expression, Isolation and Analysis … We investigate the potential of C-HapS-P6 fusion protein as a … into Escherichia coli for protein expression. The mice were … (Genscript, Nanjing, China). The sizes of the soluble fusion … Get A Quote

摘要

Nontypeable Haemophilus influenzae (NTHi) is the dominant pathogen in several infectious diseases. Currently the use of antibiotics is the main intervention to prevent NTHi infections, however with the emergence of drug resistant strains, it has compromised the treatment of respiratory infections with antibiotics. Therefore there is an urgent need to develop a safe and effective vaccine to prevent NTHi infections. We investigate the potential of C-HapS-P6 fusion protein as a vaccine for treating NTHi in murine models. PGEX-6P2/C-HapS-P6 fusion gene was constructed using overlap extension polymerase chain reaction. The recombined plasmid was transformed into Escherichia coli for protein expression. The mice were... More

關鍵詞

C-HapS-P6 fusion protein, Immunogenicity, Nontypeable Haemophilus influenzae infection, Vaccine
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