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Selective Detection of Active Extracellular Granzyme A by Using a Novel Fluorescent Immunoprobe with Application to Inflammatory Diseases

ACS Pharmacol Transl Sci. 2024-04; 
Ana Senan-Salinas, Laura Comas, Patricia Esteban, Marcela Garzón-Titua?a, Zhiming Cheng, Llipsy Santiago, Maria Pilar Domingo, Ariel Ramírez-Labrada, José Ramón Pa?o-Pardo, Marc Vendrell, Julián Pardo, Maykel A Arias, Eva M Galvez
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Proteins, Expression, Isolation and Analysis … The DNA sequences encoding amino acid residues of the mouse and human GzmA were synthesized by GenScript (USA) for expression in Pichia pastoris using the protein expression … Get A Quote

摘要

Granzymes (Gzms), a family of serine proteases, expressed by immune and nonimmune cells, present perforin-dependent and independent intracellular and extracellular functions. When released in the extracellular space, GzmA, with trypsin-like activity, is involved in the pathophysiology of different inflammatory diseases. However, there are no validated specific systems to detect active forms of extracellular GzmA, making it difficult to assess its biological relevance and potential use as a biomarker. Here, we have developed fluorescence-energy resonance-transfer (FRET)-based peptide probes (FAM-peptide-DABCYL) to specifically detect GzmA activity in tissue samples and biological fluids in both mouse and human s... More

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