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MicroRNA-223-3p downregulates the inflammatory response in preeclampsia placenta via targeting NLRP3

BMC Pregnancy Childbirth. 2024-03; 
Xueqiong Liu, Zhiyue Li, Dan Lu
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Gene Synthesis … Finally, primers were designed and then synthesized by GenScript Biotechnology Ltd (Jiangsu, Nanjing, China), as shown in Table 1. Then the reaction liquid mixed according to the … Get A Quote

摘要

objective: To investigate the regulatory role of miR-223-3p in the inflammatory response of PE placenta. methods: PE and normal placental tissues were collected to measure the expression of NLRP3 and miR-223-3p. The targeting relationship between NLRP3 and miR-223-3P was verified by bioinformatics analysis and classical double-luciferase reporter gene assay. Lipopolysaccharide (LPS) was used to induce HTR8/SVneo cells as PE placental cell inflammation model. Then we transfected miR-223-3p overexpression/miR-223-3p negative control plasmid into the LPS-induced HTR8/SVneo cells. Next, the expressions of NLRP3, Caspase-1, GSDMD, IL-1β and IL-18 were evaluated to elucidate the regulatory effect of miR-223-3p on th... More

關(guān)鍵詞

NLRP3 inflammasome, Placenta inflammatory dysregulation, Preeclampsia, microRNA-223-3p
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