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Purification and biochemical characterization of a novel chitosanase cloned from the gene of Kitasatospora setae KM-6054 and its application in the production of chitooligosaccharides

World J Microbiol Biotechnol. 2023-03;

Yinxiao Xu, Hui Wang, Benwei Zhu, Zhong Yao, Ling Jiang

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Proteins, Expression, Isolation and Analysis	… The crude enzyme solution was then purified using Ni-charged magnetic beads (GenScript, Nanjing, China). The heteroproteins were eluted with Tris–HCl buffer (pH 8.0) containing 20 …	Get A Quote

摘要

Chitosanase could degrade chitosan efficiently under mild conditions to prepare chitosan oligosaccharides (COSs). COS possesses versatile physiological activities and has wide application prospects in food, pharmaceutical and cosmetic fields. Herein, a new glycoside hydrolase (GH) family 46 chitosanase (CscB) was cloned from Kitasatospora setae KM-6054 and heterologously expressed in Escherichia coli. The recombinant chitosanase CscB was purified by Ni-charged magnetic beads and showed a relative molecular weight of 29.19?kDa by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). CscB showed the maximal activity (1094.21 U/mg) at pH 6.0 and 30?°C. It was revealed that CscB is a cold-adapted... More