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Heteromultivalent scaffolds fabricated by biomimetic co-assembly of DNA-RNA building blocks for the multi-analysis of miRNAs

J Mater Chem B. 2023-02; 
Chengjie Duan, Yan Chen, Zhiqiang Hou, Dayong Li, Jin Jiao, Weihao Sun, Yang Xiang
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Proteins, Expression, Isolation and Analysis … RNA sequences were purchased from Genscript Biotech … of Nanjing Medical University. The study was approved by the ethics committee of Nanjing Medical University and Nanjing … Get A Quote

摘要

Heteromultivalent scaffolds with different repeated monomers have great potential in biomedicine, but convenient construction strategies for integrating various functional modules to achieve multiple biological functions are still lacking. Here, taking advantage of the heteromultivalent effect of dendritic nucleic acids and the specific biochemical properties of microRNAs (miRNAs), we assembled novel heteromultivalent nucleic acid scaffolds by biomimetic co-assembly of DNA-RNA building blocks. In our approach, two miRNAs were used to initiate and maintain dendritic structures in an interdependent manner; so, the heteromultivalent nanostructure can only form in the presence of both miRNAs. The proposed nanostruc... More

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